Aim
To identify the stages of gamete development, i.e., T.S. of testes and ovary through permanent slides from mice.
Materials Required
- Permanent slides of T.S. of testes.
- Permanent slides of T.S. of the ovary.
Procedure
T.S. of Testes of Mice
- The testes comprise several seminiferous tubules embedded in the interstitial tissues.
- Thick fibrous tissues called tunica albuginea cover the testes.
- It comprises different types of cells from the outside to the lunar in the manner given below:
Spermatogonia → Spermatocytes → Spermatids → Spermatozoa (sperms)
- Sertoli cells are located between the germinal cells.
- The Leydig cells that produce testosterone are present in the interstitial tissues.
T.S. of Ovary of Mice
- An ovary is a germinal epithelium bounded by a solid structure covered by a thick layer of fibrous tissue known as tunica albuginea.
- It consists of an inner medulla and an outer cortex.
- The medulla comprises several round or oval bodies known as ovarian follicles.
- Follicle development takes place in the following stages:
1°follicle → 2°follicle → 3°follicle → Graffian follicle → Corpus luteum
- Cortex comprises corpus luteum along with mature follicles.
Precautions
- The microscope should be handled with care.
- Adjust the lens such that the focus is better.
Learn more in detail about the stages of gamete development, other related topics and experiments at CoolGyan’S Biology.