The formula for retention time is given as

k = $\frac{t_{R} – t_{o}}{t_{o}}$ = $\frac{t_{R}}{t_{o}}$.

According to the retention factor, inert tracer which are not absorbed is zero.

According to the retention factor the non absorbed inert tracer is zero. The retention factor has various significance as the most significant quantity that can be derived directly from the chromatogram. It is a partition without dimension or the distribution ratio given by.

k = $\frac{amount~ of~ an~ eluite~ in~ the~ stationary~ phase}{amount ~of~ that~ eluite~ in~ the~ mobile~ phase}$.

## Retention Factor Formula Problems

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**Question1:If a 125 $\times$ 4.6 mm column and a flow rate of 1 mL min ^{-1}. Calculate the estimated dead time.**

**Solution: **

t_{M} = 0.1 $\times$ $\frac{12.5}{1}$ = 1.25

k = $\frac{t_{R}-t_{M}}{t_{M}}$ applies

Solving the equation for t_{R} yields

t_{R} = (t_{M} + 1)k and thus t_{R} = (1.25 + 1)4

**t _{R }=**

**9min**