Identification of Stages of Gamete Development, i.e. T.S of Testes and T.S of Ovary through Permanent Slides from Grasshopper Mice
The aim of the transverse section of ovary and testis is to identify the development of gametes with the help of permanent slides.
Tools Required for both TS of Mammalian Testis and Ovaries are Mentioned Below:
- Compound microscope.
- Permanent slides of T.S of mammalian ovary and testis.
- Lens clearing papers.
- Cleaning fluid.
Let’s proceed with the process of conducting TS of mammalian ovary and testis.
- First, wash hands properly and dry them thoroughly.
- Then pour some cleaning fluid on the lens cleaning papers. Wipe slides, the microscope’s eye and objective lenses.
- Next, place both slides one by one on the microscope’s platform. Observe the slides under lower magnification and then higher one.
- Remember the stages of the development of gametes.
- Write down your observations in biology practical copy. Now draw diagrams of both TS of mammalian ovary and testis and label them.
Transverse Section of Mammalian Ovary
Ovaries are the primary reproductive organs of almost all female vertebrates. The main function of them is to produce female gametes or eggs. Also, they are responsible for forming progesterone hormones.
Most mammals, including female humans, possess two ovaries, right and left.
The development of gametes inside ovaries is called oogenesis.
TS of mammalian ovaries will help you to study the several components of ovaries.
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TS of mammalian ovary under microscope reveal the following aspects.
- First, there is a Germinal Epithelium that covers a group of tissues. An ovum, that is a cell remains inside it. Furthermore, one or several layers of follicular cell guard the ovum. The number of follicular cell layers increases as the ovum matures.
- The next stage shows that antrum, a cavity appears.
- Further, the cavity grows longer, and follicle gets bigger. During this stage, Graffian Follicle can release ovum. This process is called ovulation.
- In later stages, a Corpus Luteum and Corpus Albicans may appear. However, they have different characteristics. They also differ from the follicular cell. Corpus Luteum forms from the ruptured follicle that contains Lutein cells and surrounded by blood clots.
- Atretic follicle shows that the follicle cells are disorganised. It also carries the sign of nuclear necrosis. Moreover, it also shows the reduced size of the oocyte.
All these stages are clearly seen in permanent ovary slide.
Transverse Section of Mammalian Testis
The testis is the most vital male reproduction organ. It is responsible for producing male gametes or sperms and storing them. Also, it forms testosterone hormones that influence sex drive, fertility and increases bone mass and muscle power.
Male humans have a pair of the testis. The process of gametogenic development in testes is known as spermatogenesis.
TS of testis under microscope shows these components.
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- There are many Seminiferous tubules. They are somewhat round or oval.
- TS of mammalian testis under microscope shows a few cells like Primary Spermatocytes, Secondary Spermatocytes, Germinal Epithelium, Spermatozoa and Spermatids.
- A permanent testis slide shows that in between tubules, there are blood vessels and a special type of cell named Leydig’s cells.
- Also, you can see Sertoli cells that hold the sperms.
- However, the mature sperms remain inside the lumen of the Seminiferous tubule. In this stage, the sperm has a head and tell, but the middle is still immature.
Fill in the gap: Sertoli cells are separated by _______________ cells.
Now, draw TS of mammalian testis diagram and label it.
FAQ (Frequently Asked Questions)
- What type of Mammals have Ovaries?
Ans. Almost all female vertebrates have ovaries. However, most Platypuses and birds do not have a mature right ovary. Only their left ovary is functional.
- What is the Difference Between Testis and Ovary?
Ans. Testis is the primary male reproductive organ, while ovary is the main female reproductive organ. Testis and ovary form testosterone and progesterone hormones, respectively.
- What is Corpus Luteum?
Ans. Corpus Luteum is a group of cells that forms inside an ovary. During the early phase of pregnancy, it releases progesterone hormones.