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Difference Between Gram-Positive and Negative Cell Wall

The cell wall is the outermost and non-living part of the cell. It is present only in plant cells and also seen in some fungi, bacteria and algae. The cell wall is a thick, rigid, semi-permeable membrane and has elastic properties that help them to grow in thickness over the period of time. The cell wall is metabolically inactive as it lacks receptors and is mainly involved in:

  1. Providing a fixed shape to the cell.
  2. Protects the cell from the external environment.
  3. Acts as a barrier between the external and internal environment.

Also Refer: Cell wall

Both, the Gram-positive and Gram-negative bacteria are the genus of bacteria family and a member of the phylum Firmicutes.

Gram-positive bacteria are the group of a heterogeneous bacteria, which appear purple in colour when they are stained in the crystal violet dye and examined under a microscope. Clostridium, Actinomyces, Mycobacterium, Nocardia streptococci, and staphylococci, are a few examples of gram-positive bacteria.

Gram-negative bacteria are the group of aerobic bacteria, which appear pink in colour when they are stained in the crystal violet dye and examined under a microscope. Vibrio, Campylobacter, Pseudomonas and members of the family Enterobacteriaceae are a few examples of gram-negative bacteria.

Also Read- Difference Between Gram-positive and Gram-negative Bacteria

Here, let us explore more information about the difference between gram-positive and negative cell wall.

Cell Wall of Gram-positive and Gram-negative Bacteria – Differences

Difference between gram positive and negative cell wall

Gram-Positive Cell Wall Vs Gram-Negative Cell Wall

Gram-Positive Cell WallGram-Negative Cell Wall
Layers
Single layeredDouble layered
Composition
Consisting of several layers of peptidoglycanConsisting of a single layer of peptidoglycan
Thickness
15 to 80 nanometers10 nanometers
Periplasmic space
SmallLarge
Outer membrane
AbsentPresent
Teichoic Acid
PresentAbsent
Porins
AbsentPresent
Lipid Content
Very low (2 – 5 per cent)High (15-20 per cent)
Lipopolysaccharides
AbsentPresent
Gram staining Procedure
Retains the crystal violet dye and appear purple in colourDoes not retain the crystal violet dye and appear pink in colour

These differences between the Cell Wall of Gram-positive and Gram-negative Bacteria are classified based on their structure, composition of the cell and by the procedure of Gram staining technique.

This was a brief introduction to the difference between gram-positive and negative cell wall. Stay tuned with CoolGyan’S to learn more in detail about the various topics like Bacteria and Bacterial Diseases at CoolGyan’S Biology.